Protein Purification
Zymo Research Corporation Y-Lysis Buffer, 6 ml
Y-Lysis Buffer (6ml) for use with the Yeast Protein Kit for rapid lysis of yeast cells. For use with Zymo Research nucleic acid purification kits for DNA or RNA.
IBA LifeSciences Strep-TactinXT PE , 50 µl
Strep-TactinXT conjugated to R-Phycoerythrin (PE) is optimized for flow cytometry of cells. It binds to Strep-tagII- or Twin-Strep-tag- fusion proteins with a high affinity.Cells can either be stained directly if they express a strep-tagged surface protein or indirectly via a strep-tagged protein that subsequently binds to the cells (e.g. for sorting antigen-specific B cells). Independent from the chosen method, biotin addition will cause the release of Strep-TactinXT PE from the cell surface. This enables the use of selected cells for further downstream applications.This conjugate is especially recommended for low-affinity or low-expressed targets.
Gold Biotechnology Inc Nickel NTA Agarose Beads 100 mL
Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.
Dojindo Molecular Technologies Inc DOJINDO MOLECULAR TECHNOLOGIES INC
NC3986758 EXOSOME PROTEIN FLUOR STAINING
Showa Denko America Inc SHODEX OHPAK SB-807 HQ COLUMN
Polyhydroxymethacrylate columns for aqueous SEC (GFC) analysis. The eluent can be replaced with DMF for the analysis of polar polymers. Good replacement for protein analysis when silica cannot be used. Pullulan exclusion limit: 500,000,000
STEMCELL Technologies EasySep™ Human Monocyte Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Enrichment Kit is designed to isolate monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to prevent nonspecific binding of monocytes. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which CD16+ cells are not removed, we recommend the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058). For even faster cell isolations, we recommend the new EasySep™ Human Monocyte Isolation Kit (19359) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-Monocyte-Isolation-Kit.aspx) which isolates cells in as little as 12.5 minutes.
STEMCELL Technologies EasySep™ Direct Human Total Lymphocyte Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Total Lymphocyte Isolation Kit isolates highly purified lymphocytes directly from human whole blood by immunomagnetic negative selection. Isolated cells are untouched and available for direct use in flow cytometry, culture, DNA/RNA extraction or other downstream applications.
Biotage GEL FILTRATION PHYTIP COLUMNS
Gel filtration PhyTip® columns (1000 µL volume) containing 600 µL of 5K size exclusion resin per column
Supply Solutions Protease Inhibitor Cocktail, MFCD00677817, 1mL
For use with mammalian cell and tissue extracts, DMSO solution, Inhibits serine, cysteine, and acid proteases, and aminopeptidases.
STEMCELL Technologies EasySep™ Human B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Isolation Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched, desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human B Cell Enrichment Kit (Catalog #19054) for even faster cell isolations.
Abcam CHAPS Detergent, 10% Solution, Sterile-Filtered
A zwitterionic detergent
The product is subject to the following: Abcam Restricted Use Statement